PureLink™Pro 96 Viral RNA⁄DNA Purification Kit is a purification kit from 200 µL of A 96-well silica gel plate system for extracting viral RNA and DNA from cell fluids. This system uses a low-depth 96-well centrifuge basket, which is simple and easy to use without losing sensitivity. These kits offer the following features:
• Versatility - just one kit to process all your samples, with protocols optimized for viral RNA and DNA extraction
• Flexible centrifuge selection - for The choice of a minimum processing speed of 2250 xg for 96-well plate processing and the use of a rotor with a basket depth of 5 cm make this kit one of the more suitable systems for existing laboratory centrifuges (Figure 1)
• Sensitivity and reproducibility as spin columns - high throughput, reliable, easy to use without sacrificing quantitative accuracy

PureLink™Pro 96 Viral RNA⁄DNA Purification The kit is based on traditional silica gel plate extraction chemical reagents. Cell-free samples are dissolved in reconstituted guanidine-containing buffer to increase recovery and sensitivity of purified nucleic acids, while carrier RNA is used to protect samples from RNase degradation. Binding of lysates to viral filter plates can be performed using a vacuum manifold or centrifugation using a 96-well plate rotor (basket depth only 5 cm). After washing with ethanol buffer, viral RNA or DNA is eluted in 100 µL of RNase-free water. RNA or DNA can be used directly in one- or two-step RT-PCR as well as qRT-PCR, qPCR or other enzymatic amplification procedures.

PureLink™Pro 96 Viral RNA⁄DNA Purification Kit Shows Excellent Well-to-Well Consistency
A key requirement for any 96-well plate purification system is well-to-well consistency Have consistency. When using a technology as sensitive as qRT-PCR for viral nucleic acid detection, consistency between samples becomes even more important if viral titers in patient samples are low. When analyzing well-to-well recovery of lentiviral RNA using the PureLink™Pro 96 Viral RNA⁄DNA Purification Kit, consistent Ct values were obtained in qRT-PCR analysis, whereas plasma controls on the same plate The product was negative, indicating no cross-contamination (Figure 2).

Use spin columns to get the same results as you do in 96-well plate extractions
Proven that our new purification chemistry delivers consistency in both high and low throughput formats, Armor RNA™ HCV virus is added to human plasma and 200 µL is used for viral nucleic acid purification. PureLink™ Viral RNA⁄DNA Mini Kit (spin column-based purification) or PureLink™Pro 96 Viral RNA⁄DNA purification can be used. Reagent test kit. Purified samples were analyzed for performance comparison using qRT-PCR. Both purification methods successfully demonstrated virus purification and detection, as both purification profiles showed scalable qRT-PCR assay profiles (Figure 3). PureLink™Pro96 Viral RNA⁄DNA Purification Kit provides sensitive, linearly scalable viral RNA⁄DNA purification with the same sensitivity and efficiency as spin column purification specifications.

Improved Plate Design
PureLink™Pro Viral RNA⁄DNA Purification Kit offers an optimized skirted 96-well plate (Figure 4) for improved performance and results. In addition, the plate nozzle containing an annular collar prevents sample cross-contamination and improves drying of the silica membrane, thereby preventing ethanol residue. Alternatively, samples can be processed manually using a benchtop vacuum manifold, via centrifugation (at 2250 xg) or via an automated liquid handling platform. The semi-skirted panel design is compatible with most vacuum manifolds on automated workstations.